The microphysiometer assay system is the most generic assay available for measuring the metabolic activation of cells expressing GPCRs following activation by an agonist. The cellular acidification rate response induced by agonists can be monitored by a cytosensor microphysiometer. The cytosensor system uses a silicon-based light addressable potential sensor (LAPS) to monitor changes in pH. Differences in pH under basal, stimulated conditions due to an agonist and inhibited stage caused by an antagonist can be monitored with this system. The major disadvantage of this format is very low throughput.80
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