Contents

Abstract 143

8.1 Introduction 144

8.1.1 Evaluation of Various Expression Systems in Parallel 144

8.1.2 Modification of Coding Regions of Target GPCR Genes 144

8.1.3 Engineering of Fusion Proteins 144

8.1.4 Receptor Production for Structural Biology Applications 145

8.2 Cell-Free Translation 145

8.3 E. coli Expression 146

8.4 Other Prokaryotic Systems 147

8.5 Yeast Expression 148

8.6 Insect Cells 149

8.7 Mammalian Expression 150

8.8 Comparison of Expression Systems 151

8.9 Conclusions 154

References 154

0 0

Post a comment