Effects of SCF on Primitive Progenitor Cells In Vitro

In liquid cultures of bone marrow-derived primitive progenitors (of varying purity), SCF alone generally does little more than maintain survival of c-kit+ cells identified as primitive progenitors by the criteria referred to in Subheadings 7.2. and 7.3. (4,5,7).

However, along with various combinations of other relatively early-acting cytokines (such as interleukin [IL]-3, IL-6, or granulocyte-macrophage [GM] CSF), SCF can promote the expansion of such cells. Studies include assessments of bone marrow-derived or cord blood-derived LTR (67-70), CFU-S12 (67), pre-CFC (71), HPP-CFC (72), blast cells (57), multipotent cells (73,74), LTC-IC/extended(E)-LTC-IC (70,74), and cells expressing relevant cell surface markers (67).

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