CKit Expression by Primitive Hematopoietic Cells and Progenitors Human

Since the mid-1980s, a major criterion for identification/selection of human hematopoietic stem and progenitor cells has been cell surface expression of the CD34 marker (although it has been proposed that a small CD34- population may be more primitive and may have LTR [63]). Approximately 1-4% of bone marrow cells are CD34+, and 0.01% of (unmobilized) peripheral blood cells are CD34+. As in the mouse, c-kit has proved to be an important marker also. Approximately 1-4% of bone marrow cells, 1-4% of fetal liver cells, and 0.1% of (unmobilized) nonadherent peripheral blood cells are c-kit+ (4,5,7,8). In the bone marrow, most CD34+ cells (approx 75%) are also c-kit+, and in the peripheral blood, about 50% of CD34+ cells are also c-kit+. Cells with immature blast-like morphology predominate in sorted CD34+c-kit+ populations. As a recent example of enrichment for primitive cells, CD34+CD38-CD33-Rho123lowc-kit+ cord blood cells were shown to be highly enriched in long-term culture-initiating cells (LTC-ICs) and myeloid-lymphoid initiating cells (ML-ICs) (64). Committed hematopoietic progenitors are clearly c-kit+; in vitro, blocking Ab against c-kit inhibits the formation of CFU-C (including erythroid burst-forming units (BFU-Es), granulocyte-macrophage colony-forming units (CFU-GMs), macrophage CFUs (CFU-Ms), and mixed CFUs (CFU-Mix). In semisolid cultures (4,65). As in the mouse, it has been proposed that the most primitive stem cells may be c-kitlow or c-kit<low/c-kit- (60), and there is evidence that very primitive hematopoietic progenitors are not totally SCF/c-kit-dependent (66).

Was this article helpful?

0 0
10 Ways To Fight Off Cancer

10 Ways To Fight Off Cancer

Learning About 10 Ways Fight Off Cancer Can Have Amazing Benefits For Your Life The Best Tips On How To Keep This Killer At Bay Discovering that you or a loved one has cancer can be utterly terrifying. All the same, once you comprehend the causes of cancer and learn how to reverse those causes, you or your loved one may have more than a fighting chance of beating out cancer.

Get My Free Ebook

Post a comment