InVivo Studies in Animal Models

Drug metabolism studies in animal models provides important information concerning the structures of metabolites before embarking on costly human studies. MS is used to provide confirmatory information if synthetic metabolites are available and to help in the structural characterization of novel metabolites. Many studies have employed off-line HPLC purification of urinary metabolites followed by mass spectral analysis. Surprisingly, in view of the recognized utility of soft ionization...

Qualitative And Quantitative Analyses

The ultraviolet-visible spectra of most compounds are of limited value for qualitative analysis and have been largely superseded by the more definitive infrared and mass spectroscopies. Qualitative analytical use of ultraviolet-visible spectra has largely involved describing compounds in terms of the positions and molar absorptivities of their absorption maxima, occasionally including their absorption minima. Indeed, some organic compounds are still characterized in terms of the number of peaks...

References

Quantitative aspects of the reaction between insulin and insulin-binding antibody. J Clin Invest 38 1996, 1959. 2. BD Albertson, FP Haseltine. Non-radiometric Assays, Technology and Application in Polypeptide and Steriod Hormone Detection. New York Alan R. Liss, 1988. 3. DS Skelley, LP Brown, PK Besch. Radioimmunoassay. Clin Chem 19 146, 1973. 4. GC Oliver, BM Parker, DL Brasfield, CW Parker. The measurement of digitoxin in human serum by radioimmunoassay. J Clin Invest 47...

InVivo Studies in Humans

Human drug metabolism studies have made extensive use of EI methodology (206,207). The uricosuric drug benzbromarone was found to be extensively metabolized in humans, forming two major metabolites (206). The metabolites were 1'-hydroxybenzbromarone and 6-hydroxybenzbromarone. Benzbromarone was hydroxylated in vivo at the prochiral center C1' to 1'-hydroxybenzbromarone. Analysis of 1'-hydroxybenzbromarone from plasma and urine extracts by chiral HPLC revealed that two peaks were eluted which...

Collisioninduced Dissociation

CID requires the tandem pairing of two mass analyzers tandem mass spectrometers . In this technique, an ion of one particular mass is allowed to pass through the first analyzer into a field-free region, where it collides with an inert gas to form product product ions. The product ions are then separated in the second analyzer, which in turn transmits them to the detector 4,35 . The type of instrumentation used in these studies can be a triple-stage quadrupole mass spectrometer, a four-sector...