Using RAPD-PCR fingerprinting, the detection of mutations induced by estrogen (E) exposure was studied in nonmalignant- and malignant cells. Cells exposed to 17a-and 17 (3-estradiol, diethylstilbestrol, bisphenol A, and a-zearalanol induced mutations in different regions of the genome. They consisted of insertion/deletion as a result ofpoint or length mutations and quantitative changes as a result ofhypoploidy or polyploidy. We also detected several mutated loci in tumor-free tissues, adjacent to tumors, and DES-induced tumors. The data suggest that these mutations occurred in early stages of stilbene E-induced renal carcinogenesis, and that they may be induced as a result of stilbene E treatment.
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