In tested prostate cancer cell lines PTEN blocked the cell cycle GrS transition in a p53 and pRb independent manner. These data are not in agreement with already published mechanisms in some breast cancer cells. Protein phosphatase activity is essential for the PTEN cell cycle effect in both prostate cancer cell lines. The

Figure 2. Western blot analysis of protein expression involved in cell cycle and PI3-K/PKB/Akt pathway regulation in exponentially growing DU-145 and PC-3 cells

protein and lipid phosphatase activity are essential for apoptosis induction in

DU-145 cells. The inverse correlation between PTEN and phosphorylated

PKB/Akt supports their opposite role in the PI3-K/PKB/Akt pathway.

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