Unopette White Blood Cellplatelet Count

Principle

The Unopette system is a system of prefilled blood dilution vials containing solutions that will preserve certain cell types while lysing others. Capillary pipettes are available to draw up different volumes of blood. The dilution is determined by the type of capillary used. The diluted blood is added to a hematocytometer chamber,

Table 20.4 O Qualitative Grading of RBC Morphology

Grade Degree of Abnormalities

1 to 5 cells/10 fields Slight

6 to 15 cells/10 fields Moderate

> 15 cells/10 fields Marked

Table 20.5 O Red Cell Morphologies Matched to Clinical Conditions

RBC Morphology Relative Clinical Conditions RBC Morphology Relative Clinical Conditions

Anisocytosis Microcytes

Macrocytes

Acanthocytes

Burr cells

Ovalocytes

Severe anemias Iron deficiency Hemoglobinopathies

Megaloblastic anemias (vitamin B12 deficiency and folic acid deficiency) Pernicious anemia Folic acid deficiency Abetalipoproteinemia Neonatal hepatitis Postsplenectomy Heparin administration Cirrhosis of liver with associated hemolytic anemia

Variety of anemias Gastric carcinoma Peptic ulcers Renal insufficiency Uremia

Pyruvate kinase deficiency Hemoglobin C disease Hemolytic anemias Hereditary ovalocytosis Iron deficiency anemia Megaloblastic anemia Pernicious anemia Sickle cell trait Thalassemia

Schistocytes

Sickle cells Spherocytes

Stomatocyte

Target cells

Teardrops

Hemolytic anemias related to burns and prosthetic implants Renal transplant patients Disseminated intravascular coagulation (DIC) Hemolytic uremic syndrome (HUS) Thrombotic thrombocytopenic purpura (TTP) Sickle cell anemia

ABO incompatibility DIC

Bacterial toxins Hemolytic anemias Blood transfusion reactions Congential spherocytosis Alcoholism

Hereditary spherocytosis Infectious mononucleosis Lead poisoning

Liver disease including cirrhosis

Malignancies

Thalassemia minor

Hemoglobinopathies: HbC disease, S-C, S-S Sickle cell thalassemia Hemolytic anemias Iron deficiency

Liver disease including cirrhosis Postsplenectomy Myeloproliferative syndromes Severe anemias

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