Lori Lentowski and Betty Ciesla

Microhematocrit

Principle

Reagents and Equipment

Specimen Collection and Storage

Quality Control

Procedure

Interpretation

Calculating Red Blood Cell Indices

Normal Average Values

Modified Westergren Sedimentation Rate

Principle

Reagents and Equipment

Specimen Collection and Storage

Quality Control

Procedure

Normal Ranges

Limitations

Conditions Associated With...

Manual Reticulocyte Procedure

Principle

Reagents and Equipment Specimen Collection and Storage Quality Control Procedure Normal Values

Conditions Associated With. Limitations

Reticulocyte Procedure With Miller Eye Disc

Principle

Reagents and Equipment Specimen Collection and Storage Quality Control

Procedure Normal Values

Conditions Associated With. Limitations

Peripheral Smear Procedure

Principle

Reagents and Equipment

Specimen Collection and Storage

Quality Control

Procedure

Limitations

Performing a Manual Differential and Assessing Red Blood Cell Morphology

Principle

Reagents and Equipment Specimen Collection and Storage Quality Control Procedure

Unopette White Blood Cell/Platelet Count

Principle

Reagents and Equipment Specimen Collection and Storage Quality Control Procedure

Cell Counts and Calculations Normal Values Limitations

Sickle Cell Procedure

Principle

Reagents and Equipment Specimen Collection and Storage Quality Control

298 PartV • Laboratory Procedures

Procedure

Interpretation of Results and Result Reporting Limitations

Cerebrospinal Fluid/Body Fluid Cell Count and Differential

Principle

Reagents and Equipment Specimen Collection and Storage Quality Control Procedure

Prothrombin Time and Activated Partial Thromboplastin Time: Automated Procedure

Principle

Reagents and Equipment

Specimen Collection and Storage

Quality Control

Procedure

Results

Limitations

The following procedures are representative of basic methods employed in hematology laboratories and have been written in Standard Operating Procedure (SOP) format.

We hope they will provide a ready reference and give students the opportunity to preview how a procedure would be introduced into the clinical setting. In addition to the SOPs, specific manufacturer's instructions on instrumentation and reagents would be strictly followed in a working clinical laboratory

Information on scatterplots and flow cytometry is presented at the end of this chapter. This information is fairly basic and serves only to kindle the interest of the student and expose them to this subject matter. No attempt has been made to create comprehensive coverage of these areas.

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