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RBC/WBC Inclusion

Disease Association

Auer rods

Basophilic stippling

RBC/WBC Inclusion

Disease Association

Auer rods

Basophilic stippling

Cabot rings

Döhle bodies

Hemoglobin C Heinz bodies Howell-Jolly bodies

Pappenheimer bodies

Toxic granulation

Acute myeloid leukemias

Lead poisoning Arsenic poisoning Severe anemias Sideroblastic anemia Hemolytic anemia Unstable hemoglobin Pyrimidine 5'-nucleotidase deficiency Lead poisoning Pernicious anemia

Infection Burn patients Hemoglobin C disease G6PD deficiency Hemolytic anemias Sickle cell anemia Pernicious anemia Postsplenectomy Megaloblastic anemia Lead poisoning

Iron loading anemias Sideroblastic anemia Postsplenectomy Lead poisoning Thalassemia Infections Burn patients Drug therapy

Reagents and Equipment

1. Unopette reservoirs containing ammonium oxalate diluent. Check expiration dates and do not use expired Unopettes. Protect from sunlight. Storage temperature is 1 to 30oC.

2. Unopette capillary pipette, 20 pL.

3. Hematocytometer: improved Neubauer ruling

4. Hematocytometer coverslips

5. Petri dish lined with filter paper that has been moistened and two applicator sticks to hold the hematocytometer

6. Microscope with phase

7. Hand counter

Quality Control

1. All WBC and platelet counts are done in duplicate. WBC counts should agree ±20%. Platelet counts must agree ±10%. If they do not agree, repeat counts.

2. A visual estimate of the white count and the platelets can be done on the peripheral smear. Refer to charts.

3. Laboratory professionals are trained and tested for proficiency. Results are documented in their training file.

Specimen Collection and Storage

Specimen of choice is a Microtainer EDTA or EDTA

tube, which should be at least half full.

Procedure

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