Features Of Cell Identification

Descriptions for this section represent composite criteria for each cell identification.3-5 In addition to key distinguishing features, differentiating characteristics are presented for most cells.

Myeloblast

Size: 12 to 20 pm

N:C: 4:1 with round, oval, or slightly indented nucleus

Chromatin: Light red-purple with a fine meshlike and transparent structure/close-weaved texture; may see two to five nucleoli, which appear as lightened, refractile round structures

Cytoplasm: Moderate blue and usually nongranu-lar

Differentiating characteristic: Nucleus has thin chro-matin strands that are distributed throughout the nucleus uniformly; chromatin appears smooth and velvety (Fig. 9.1).

Cluster designation (CD)45, CD38, CD34, CD33, CD13, human leukocyte antigen (HLA)-DR

Promyelocyte (Progranulocyte)

Size: 15 to 21 pm

N:C: 3:1, oval, round, or eccentric flattened nucleus

Chromatin: Light red-purple of medium density, may see single nucleoli

Cytoplasm: Moderate blue color but difficult to observe because fine to large blue-red azurophilic granules are scattered throughout the chromatin pattern; granules are NONSPECIFIC

Differentiating characteristic: Cell is larger than the blast with large prominent nucleoli, nuclear chromatin is slightly coarse (Fig. 9.2).

CD45, CD33, CD13, CD15

Myelocyte

Size: 10 to 18 pm

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