1. This approach is robust against false negatives. Each biological function is usually represented by several probesets. For example, if 11 probesets represent a biological function and five of them are captured by the list, the biological function is

Fig. 3.28 Biological pathways may agree between lists, even if the actual probesets vary.

still indicated. If the second list captures six probesets the function is still indicated even if none of these probesets are common between the lists (Figure 3.28).

2. This approach is robust against false positives. The GO viewer (discussed elsewhere) allows the operator to set a minimum number of probesets that must be mapped to a function before the function is displayed. Random false positives are expected to be scattered among random functions so that this minimum requirement is not met and the false positives are not displayed.

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