Tomara J Fleury Alfred Cioffi and Arnold Stein


Nucleosome arrangements and possibly chromatin higher order structures differ in different regions of genomic DNA, and these differences could be functionally important. Nucleosome arrangements are reflected by the nucleosome ladders they give rise to upon micrococcal nuclease digestion of the chromatin. Here we describe how Southern hybridization can be used to detect the nucleosome ladders arising from different regions of DNA in mouse liver nuclei.

Key Words: Nucleosome ladders; chromatin; mouse liver nuclei; genomic DNA; Southern hybridization.

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