The rapid advances of genome sequencing projects have offered the opportunity to map and locate genes of interest, without resorting to time-consuming and costly experimental procedures. Large sequencing projects routinely deposit their data in publicly available databases including GenBank, at the National Center for Biotechnology Information (NCBI) in the United States (1,2); EMBL, maintained by the European Bioinformatics Institute (3); and DDBJ, the DNA Data Bank of Japan (4).

Currently, several browsers offer facile access to numerous genomic DNA sequences for gene mapping and data retrieval. These include NCBI (1,2); the genome browser at the University of California at Santa Cruz (UCSC) (5,6); and the browser maintained by Ensembl (7). All three browsers offer sophisticated

From: Methods in Molecular Biology, vol. 338: Gene Mapping, Discovery, and Expression: Methods and Protocols Edited by: M. Bina © Humana Press Inc., Totowa, NJ

tools for gene mapping and localization on genomic DNA. This chapter provides an example of how to use the genome browser at UCSC (5,6) to obtain a map and a graphical view of a known DNA sequence.

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