And Construction of High Resolution Physical Maps

1. Quantitative DNA fiber mapping experiments require only standard laboratory equipment and access to a fluorescence microscope equipped with a film or electronic camera.

2. Filters capable of excitation in single bands centered around 360, 405, 490, 555, and 637 nm and visualization in multiple bands in the vicinities of 460 nm (blue), 520 nm (green), 600 nm (red), and 680 nm (infrared) are desirable. Single-color images are collected using a CCD camera (Xilix, Hamamatsu, Vosskuehler, Photo-metrics, or similar) connected to a computer workstation (51). When images are recorded on film, standard film with a sensitivity of ASA 400 is sufficient.

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