Size Fractionation by Superose 6 Gel Filtration

Superose 6 analytical grade column HR 10 30 with a total bed volume of 24 mL. Preparative grade column XK 50 600 with a bed height of 30.3 cm, total bed volume of 589 mL (both from Amersham Biosciences, UK). Range of separation of 5000 to 5,000,000 Daltons. Ethanol (20 ) is used as preservative. The column is connected to an AKTA FLPC system (Amersham Biosciences). 2. Gel filtration calibration kit dextran blue and high molecular weight standards (Amersham Biosciences, UK). 3. Column running...