Calendula has free radical scavenging and antioxidant activity, with aqueous extracts having greater activity than methanolic extracts and antioxidant activity being related to the total phenolic content and flavonoid content (Cetkovic et al 2004).

The butanolic fraction of a calendula extract has been shown to reduce superoxide and hydroxyl radicals, suggesting a free radical scavenging effect. Lipid peroxidation in liver microsomes is also reduced (Cordova et al 2002). Isorhamnetin glycosides isolated from calendula have been shown to inhibit the activity of lipo-oxygenase (Bezakova et al 1996).

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