Scheme 16 Solid phase synthesis of a trisaccharide by the glycal assembly method.

HR-MAS experiments proved to be an ideal way of monitoring the solid support synthesis by obtaining 1H NMR, 13C NMR, and 1H-13C NMR spectra of high quality. The 1H NMR (Scheme 17) of the crude product of this synthesis showed that only one product was obtained. Spin-echo techniques were used to suppress NMR peaks derived from the polymeric matrix. Since its introduction, this technique has greatly facilitated the development of novel synthetic schemes of oligosaccha-rides and glycopeptides on a solid support.

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