C 013 and 14Galactosyltransferases

Though not widely available yet, the ^1,3-galactosyltransferase that synthesizes Gal^1 ^ 3GlcNAc (type I) from GlcNAc acceptors has been cloned and expressed [77,78]. Mapping has indicated that N-acyl analogs can be utilized as acceptors by

Figure 5 Unnatural donors and acceptors for a!,3-galactosyltransferase.

the enzyme [79]. a1,4-Galactosyltransferase from Neisseria meningitidis transfers Gal from UDP-Gal donors solely to lactose acceptors yielding globotriose [62,72]. The enzyme will transfer arabinose, 2-deoxy-, 4-deoxy-, and 6-deoxy-Gal from modified donors from 2 to 28% of the rate of UDP-Gal; however, 3-deoxy-Gal is not transferred [72]. Large-scale production of UDP-Gal and globotriose (188 g/L) has been achieved by metabolic engineering of bacteria [20].

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