Renaturation of DNA Requires Base Pair Matching

Separated strands of DNA will renature or reassociate when appropriate physiologic temperature and salt conditions are achieved. The rate of reassociation depends upon the concentration of the complementary strands. Reassociation of the two complementary DNA strands of a chromosome after DNA replication is a physiologic example of renaturation (see below). At a given temperature and salt concentration, a particular nucleic acid strand will associate tightly only with a complementary strand. Hybrid molecules will also form under appropriate conditions. For example, DNA will form a hybrid with a complementary DNA (cDNA) or with a cognate messenger RNA (mRNA; see below). When combined with gel electrophoresis techniques that separate hybrid molecules by size and radioactive labeling to provide a detectable signal, the resulting analytic techniques are called Southern (DNA/cDNA) and Northern blotting (DNA/RNA), respectively. These proce

dures allow for very specific identification of hybrids from mixtures of DNA or RNA (see Chapter 40).

Diabetes 2

Diabetes 2

Diabetes is a disease that affects the way your body uses food. Normally, your body converts sugars, starches and other foods into a form of sugar called glucose. Your body uses glucose for fuel. The cells receive the glucose through the bloodstream. They then use insulin a hormone made by the pancreas to absorb the glucose, convert it into energy, and either use it or store it for later use. Learn more...

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