Porphyrins Are Colored Fluoresce

The various porphyrinogens are colorless, whereas the various porphyrins are all colored. In the study of por-phyrins or porphyrin derivatives, the characteristic absorption spectrum that each exhibits—in both the visible and the ultraviolet regions of the spectrum—is of great value. An example is the absorption curve for a solution of porphyrin in 5% hydrochloric acid (Figure 32-10). Note particularly the sharp absorption band near 400 nm. This is a distinguishing feature of the porphin ring and is characteristic of all porphyrins regardless of the side chains present. This band is termed the Soret band after its discoverer, the French physicist Charles Soret.

When porphyrins dissolved in strong mineral acids or in organic solvents are illuminated by ultraviolet light, they emit a strong red fluorescence. This fluorescence is so characteristic that it is often used to detect small amounts of free porphyrins. The double bonds joining the pyrrole rings in the porphyrins are responsible for the characteristic absorption and fluorescence of these compounds; these double bonds are absent in the porphyrinogens.

An interesting application of the photodynamic properties of porphyrins is their possible use in the treatment of certain types of cancer, a procedure called cancer phototherapy. Tumors often take up more por-phyrins than do normal tissues. Thus, hematopor-phyrin or other related compounds are administered to a patient with an appropriate tumor. The tumor is then exposed to an argon laser, which excites the porphyrins, producing cytotoxic effects.

Spectrophotometry Is Used to Test for Porphyrins & Their Precursors

Coproporphyrins and uroporphyrins are of clinical interest because they are excreted in increased amounts in





oi pc

Four molecules of porphobilinogen

Four molecules of porphobilinogen





C H2

Type I uroporphyrinogen

Type III uroporphyrinogen

Figure 32-6. Conversion of porphobilinogen to uroporphyrinogens. Uroporphyrinogen synthase I is also called porphobilinogen (PBG) deaminase or hydroxy-methylbilane (HMB) synthase.

the porphyrias. These compounds, when present in urine or feces, can be separated from each other by extraction with appropriate solvent mixtures. They can then be identified and quantified using spectrophotometry methods.

ALA and PBG can also be measured in urine by appropriate colorimetric tests.

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Diabetes 2

Diabetes is a disease that affects the way your body uses food. Normally, your body converts sugars, starches and other foods into a form of sugar called glucose. Your body uses glucose for fuel. The cells receive the glucose through the bloodstream. They then use insulin a hormone made by the pancreas to absorb the glucose, convert it into energy, and either use it or store it for later use. Learn more...

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