Isoelectric Focusing IEF

Ionic buffers called ampholytes and an applied electric field are used to generate a pH gradient within a poly-acrylamide matrix. Applied proteins migrate until they reach the region of the matrix where the pH matches their isoelectric point (pi), the pH at which a peptide's net charge is zero. IEF is used in conjunction with SDS-PAGE for two-dimensional electrophoresis, which separates polypeptides based on pI in one dimension and based on Mr in the second (Figure 4-5). Two-dimensional electrophoresis is particularly well suited for separating the components of complex mixtures of proteins.

Diabetes 2

Diabetes 2

Diabetes is a disease that affects the way your body uses food. Normally, your body converts sugars, starches and other foods into a form of sugar called glucose. Your body uses glucose for fuel. The cells receive the glucose through the bloodstream. They then use insulin a hormone made by the pancreas to absorb the glucose, convert it into energy, and either use it or store it for later use. Learn more...

Get My Free Ebook

Post a comment